primary vaginal epithelial cells (vec-cry-ov  (MatTek)

Journal: Frontiers in Microbiology

Article Title: Recombinant Human IFNα-2b Response Promotes Vaginal Epithelial Cells Defense against Candida albicans

doi: 10.3389/fmicb.2017.00697

Figure Lengend Snippet: Effect of rhIFNα-2b on VEC viability . VK2/E6E7 cells were treated with rhIFNα-2b for 24 h. The mean values for the remaining four values and standard deviations (error bars) are shown. ∗∗∗ , significant difference compared to 0 mg/mL of rhIFNα-2b control group ( P < 0.0001).

Article Snippet: Human VEC line, VK2/E6E7 cells (ATCC ® CRL-2616), were obtained from the American Type Culture Collection (Rockville, MD, USA) and grown cultured in K-SFM (Gibco, USA) supplemented with 5 ng/mL recombinant epidermal growth factor and 50 μg/mL bovine pituitary extract (Invitrogen Corporation, Grand Island, NY, USA) with 100 units/mL each of penicillin and streptomycin (Life Technologies, Grand Island, NY, USA) at 37°C with 5% CO 2 in a high humidity environment.

Techniques: Control

Journal: Frontiers in Microbiology

Article Title: Recombinant Human IFNα-2b Response Promotes Vaginal Epithelial Cells Defense against Candida albicans

doi: 10.3389/fmicb.2017.00697

Figure Lengend Snippet: Effect of rhIFNα-2b on the production of IL-2 (A) , IL-4 (B) , IL-6 (C) , IL-8 (D) , and IL-17 (E) (expressed as pg/mL) by the VEC line, VK2/E6E7 cells cultivated alone, grown with 1.25 mg/mL rhIFNα-2b, and infected with C. albicans (1 × 10 5 /mL). The supernatants were collected and the cytokine levels were assessed by performing an ELISA 12 h post-infection and a subsequent 24 h of co-incubation with 1.25 mg/mL of rhIFNα-2b. V represents the VECs cultivated alone; V+I represents VECs co-incubated with 1.25 mg/mL of rhIFNα-2b for 24 h; V+C represents VECs infected with Candida albicans for 12 h; V+C+I represents the VECs infected with C. albicans for 12 h, then treated with 1.25 mg/mL of rhIFNα-2b for another 24 h. ∗∗ , significant difference compared to the V group ( P < 0.001); ∗∗∗ , significant difference compared to the V group ( P < 0.0001).

Article Snippet: Human VEC line, VK2/E6E7 cells (ATCC ® CRL-2616), were obtained from the American Type Culture Collection (Rockville, MD, USA) and grown cultured in K-SFM (Gibco, USA) supplemented with 5 ng/mL recombinant epidermal growth factor and 50 μg/mL bovine pituitary extract (Invitrogen Corporation, Grand Island, NY, USA) with 100 units/mL each of penicillin and streptomycin (Life Technologies, Grand Island, NY, USA) at 37°C with 5% CO 2 in a high humidity environment.

Techniques: Infection, Enzyme-linked Immunosorbent Assay, Incubation

Journal: Frontiers in Microbiology

Article Title: Recombinant Human IFNα-2b Response Promotes Vaginal Epithelial Cells Defense against Candida albicans

doi: 10.3389/fmicb.2017.00697

Figure Lengend Snippet: Effect of rhIFNα-2b on the production of vaginal epithelial-derived IgG (expressed in μg/mL) by the VEC line, VK2/E6E7 cells . Supernatants were collected, and the IgG levels were assessed by performing an ELISA after 12 h of infection and a subsequent 24 h of co-incubation with rhIFNα-2b. V represents the VECs cultivated alone; V+I represents the VECs co-incubated with 1.25 mg/mL of rhIFNα-2b for 24 h; V+C represents the VECs infected with C. albicans for 12 h; V+C+I represents the VECs infected with C. albicans for 12 h, then treated with 1.25 mg/mL of rhIFNα-2b for another 24 h. ∗∗∗ , significant difference compared to the V group ( P < 0.0001); ∗∗ , significant difference compared to the V+C group ( P = 0.004). Each sample was repeated three times. The error bars indicate the standard deviation.

Article Snippet: Human VEC line, VK2/E6E7 cells (ATCC ® CRL-2616), were obtained from the American Type Culture Collection (Rockville, MD, USA) and grown cultured in K-SFM (Gibco, USA) supplemented with 5 ng/mL recombinant epidermal growth factor and 50 μg/mL bovine pituitary extract (Invitrogen Corporation, Grand Island, NY, USA) with 100 units/mL each of penicillin and streptomycin (Life Technologies, Grand Island, NY, USA) at 37°C with 5% CO 2 in a high humidity environment.

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Infection, Incubation, Standard Deviation

Journal: Frontiers in Microbiology

Article Title: Recombinant Human IFNα-2b Response Promotes Vaginal Epithelial Cells Defense against Candida albicans

doi: 10.3389/fmicb.2017.00697

Figure Lengend Snippet: Effect of rhIFNα-2b on VEC-mediated anti-Candida activity . SEM of the control cells (A) , C. albicans infected cells at 12 h (B) , and rhIFNα-2b treated cells (C,D) . (C,D) represent the VECs infected with C. albicans for 12 h, then treated with 1.25 mg/mL rhIFNα-2b for another 24 h. Microvilli are indicated by small red arrows, filopodia are indicated with a small yellow arrow, pseudohyphae are indicated with a small blue arrow, and living VK2 cells indicated with white arrows.

Article Snippet: Human VEC line, VK2/E6E7 cells (ATCC ® CRL-2616), were obtained from the American Type Culture Collection (Rockville, MD, USA) and grown cultured in K-SFM (Gibco, USA) supplemented with 5 ng/mL recombinant epidermal growth factor and 50 μg/mL bovine pituitary extract (Invitrogen Corporation, Grand Island, NY, USA) with 100 units/mL each of penicillin and streptomycin (Life Technologies, Grand Island, NY, USA) at 37°C with 5% CO 2 in a high humidity environment.

Techniques: Activity Assay, Control, Infection

Journal: Journal of Inflammation (London, England)

Article Title: Insulin augments tumor necrosis factor-alpha stimulated expression of vascular cell adhesion molecule-1 in vascular endothelial cells

doi: 10.1186/1476-9255-8-34

Figure Lengend Snippet: Insulin and Tumor Necrosis Factor-alpha (TNFα) stimulate increases in VCAM-1 expression in vascular endothelial cells (VEC) . Cells were incubated in growth medium until 80% confluent. Growth medium was removed from cultured cells and replaced with serum-free medium for 24 h. Cells were treated without or with [A] insulin (INS) (10 nM), [B] TNFα (20 ng/mL) or [C] both for designated times and VCAM-1 protein expression was determined via Western blot analysis. Western blots are representative profiles of six assays. [D] Changes in VCAM-1 protein are expressed as the percent of control and represent the mean ± standard error of the mean (SEM) for six independent experiments. *, P < 0.05 vs controls (serum-free medium alone); **, P < 0.05 vs TNFα alone.

Article Snippet: Vascular endothelial cells (VEC) were rat aorta vascular cells purchased from ATCC (Manassas, VA) (Cat# CRL 1446) and maintained in culture medium from Gibco/Invitrogen (Carlsbad, CA).

Techniques: Expressing, Incubation, Cell Culture, Western Blot, Control